密閉消解HG-AFS法測(cè)定丹參植株中汞含量
中國(guó)測(cè)試李瑤佳， 楊 超， 胡曉榮
摘 要：為評(píng)價(jià)中江丹參植株汞含量水平、了解汞的植株分布，采用HNO3-H2O2密閉消解、氫化物發(fā)生原子熒光法測(cè)定來自中江丹參種植基地的12個(gè)植株樣品中的汞含量。實(shí)驗(yàn)優(yōu)化還原反應(yīng)條件，通過國(guó)家標(biāo)準(zhǔn)物質(zhì)比較不同消解方法對(duì)測(cè)定結(jié)果的影響及驗(yàn)證方法的準(zhǔn)確度和可靠性，密閉消解獲得結(jié)果的準(zhǔn)確度和精密度符合痕量分析要求。方法檢出限為0.12 μg/L，相對(duì)標(biāo)準(zhǔn)偏差為7.8%，樣品加標(biāo)回收率為81.0%~115.0%。丹參根中的汞含量未超過國(guó)家藥典標(biāo)準(zhǔn)（<0.2 mg/kg），部分莖和葉含量超標(biāo)，植株汞含量分布為葉>莖>根。
關(guān)鍵詞：汞；丹參；密閉消解；原子熒光
文獻(xiàn)標(biāo)志碼：A 文章編號(hào)：1674-5124（2016）01-0045-04
Determination of mercury in Salvia miltiorrhiza by HG-AFS after closed digestion
LI Yaojia， YANG Chao， HU Xiaorong
（Mineral Resources Chemistry Key Laboratory of Sichuan Higher Education Institutions，College of Materials
and Chemistry & Chemical Engineering，Chengdu University of Technology，Chengdu 610059，China）
Abstract: In order to understand the mercury level and distribution in Salvia miltiorrhiza. The total mercury concentrations in the organs of the plant collected from 12 sites across Zhongjiang were determined by hydride generation atomic fluorescence spectrometry（HG-AFS） after HNO3-H2O2 closed digestion. Reaction conditions of redox were optimized to obtain the highest sensitivity. The different analytical results of reference material digested by open and closed methods were compared. The accuracy，precision and recovery of closed digestion accorded with the requirements of trace analysis. The detection limit， relative standard deviation and recoveries rate of the method were 0.12 μg/L， 7.8%， and 81.0%-115.0% respectively. Mercury content in root was lower than the regulated value of Chinese pharmacopoeia（<0.2 mg/kg）， but it in partial leaves and stems samples exceeded the value. Mercury distribution characteristics in salvia miltiorrhiza followed concentrations order of leaf >stem>root.
Keywords: mercury； Salvia miltiorrhiza； closed digestion； atomic fluorescence spectrometry
